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online microbiology resources for allied health students


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VUMIE: Triple Sugar Iron Agar

In the identification of Gram negative bacilli, the Triple Sugar Iron Agar (TSIA) test is a trusted standard.  Not only does it provide exceptional feedback on whether the unknown microbe is a non-fermenter or enteric organism, but it also provides a wealth of information on whether gas is produced, hydrogen sulfide is generated, and whether more than one sugar is metabolized by the microbe.  Without exaggeration, it is possible to narrow the possible ID considerably with this one test.

To see how this is done, and to practice doing the test in the virtual lab, go to Micro Digital Media Exercise 10

Follow your instructor's guidance in doing this work and submitting any required lab reports.  When completed, you may be asked to take a quiz over this section.